Journal: The journal of physical chemistry. B
Article Title: Conformational Dynamics of Bacteriochlorophyll c in Chlorosomes from the bchQ Mutant of Chlorobaculum tepidum .
doi: 10.1021/acs.jpcb.4c04731
Figure Lengend Snippet: Figure 3. (a) Overlaid 13C−13C PDSD spectra of chlorosomes obtained at 25 ms mixing time at 235 K (Black) and 282 K (Red). It shows cross peaks for immobilized segments. Figure 3. (b) is a 13C−13C INEPT-TOBSY spectrum of chlorosomes from C. tepidum was obtained at 6.5 ms mixing time at 282 K, showing cross peaks for dynamic residues.
Article Snippet: 13C−13C ProtonDriven Spin Diffusion (PDSD) NMR data were collected with 256 scans for each trace in the t1 dimension, using a 13C−13C mixing time of 25 ms at temperatures of 235 and 282 K. SPINAL-64 decoupling was applied during the t2 acquisition period.34 A 13C−13C INEPT-TOtal through Bond correlation SpectroscopY, also known as (TOBSY) experiment shown in Figure 3b was performed with the sample in a 3.2 mm rotor at the magic angle in a Bruker Avance III 700 (16.4 T) spectrometer, employing a TOBSY mixing time of 6.5 ms and P961-TOBSY mixing symmetry was used.
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